Application
Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. SW40 Cells (400X, Negative)Application
Sandwich ELISA performed on the lysates of 293T cells overexpressing BRAF V600E or BRAF wild type His-tag proteins, shows the specificity of RM8 to the V600E mutation. His-tag fusion proteins were captured by an Anti-His-Tag antibody.Application
Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. WiDr Cells (400X, Positive)Application
Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein.Application
Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections.| Product Name | B-Raf (V600E Mutant Specific) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM8] to B-Raf (V600E Mutant Specific)This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | A peptide corresponding to BRAF V600E mutant |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM8 |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| Immunohistochemistry (IHC): 2 – 10 ug/mL, Immunocytochemistry (ICC): 2 – 10 ug/mL, Western Blot (WB): 0.5 – 2 ug/mL: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BRAF B-Raf proto-oncogene serine/threonine kinase NS7 BRAF1 RAFB1 B RAF1 |
|---|---|
| Target | B-Raf (V600E Mutant Specific) |
| Tissue Specificity | This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF. |
Application
Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. SW40 Cells (400X, Negative)
Application
Sandwich ELISA performed on the lysates of 293T cells overexpressing BRAF V600E or BRAF wild type His-tag proteins, shows the specificity of RM8 to the V600E mutation. His-tag fusion proteins were captured by an Anti-His-Tag antibody.
Application
Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. WiDr Cells (400X, Positive)
Application
Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein.
Application
Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections.| Application Notes | Immunohistochemistry (IHC): 2 – 10 ug/mL, Immunocytochemistry (ICC): 2 – 10 ug/mL, Western Blot (WB): 0.5 – 2 ug/mL: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
