Application
Immunofluorescence of B-raf in human small intestine tissue with B-raf antibody at 20 ug/ml.Application
Western blot analysis of B-raf in human brain tissue lysate with B-raf antibody at (A) 1 and (B) 2 ug/mL.Application
Immunohistochemistry of B-raf in human small intestine tissue with B-raf antibody at 2.5 ug/ml.| Product Name | B-Raf antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to B-RafThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | B-raf antibody was raised against a 19 amino acid peptide near the center of human B-raf. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 - 2 ug/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BRAF1 B-RAF1 RAFB1 B-Raf proto-oncogene serine/threonine kinase BRAF NS7 |
|---|---|
| Target | B-Raf |
| Tissue Specificity | At least two isoforms of B-raf are known to exist; this antibody will detect both isoforms. This antibody will not cross-react with C-raf. |
Application
Immunofluorescence of B-raf in human small intestine tissue with B-raf antibody at 20 ug/ml.
Application
Western blot analysis of B-raf in human brain tissue lysate with B-raf antibody at (A) 1 and (B) 2 ug/mL.
Application
Immunohistochemistry of B-raf in human small intestine tissue with B-raf antibody at 2.5 ug/ml.| Application Notes | WB: 1 - 2 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
