Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using HMG20B antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:2000Application
BRAF35 antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of BRAF35 by 3 μg of BRAF35 antibody 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Product Name | BRAF35 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HMG20B (high-mobility group 20B)Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BRAF35. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P,IP,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (98%), Rat (97%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BRAF25 antibody, BRAF35 antibody, FLJ26127 antibody, HMGX2 antibody, HMGXB2 antibody, PP7706 antibody, SMARCE1r antibody, SOXL antibody, pp8857 antibody, HMG20B antibody, high-mobility group 20B antibody, HMG box-containing protein 20B antibody, "SWI/SNF-r |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using HMG20B antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:2000
Application
BRAF35 antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of BRAF35 by 3 μg of BRAF35 antibody 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
