Application
BRDT antibody detects BRDT protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with BRDT antibody at a dilution of 1:500.| Product Name | BRDT antibody [C3], C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BRDT (bromodomain, testis-specific)BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BRDT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Chimpanzee, BovineHuman (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BRD6 antibody, CT9 antibody, BRDT antibody, cancer/testis antigen 9 antibody, bromodomain testis-specific protein antibody, RING3-like protein antibody, "bromodomain, testis-specific antibody" |
|---|---|
| Target | BRDT |
| Molecular Weight(MW) | 108 kDa (note) (7) |
| Cellular Localization | Nucleus |
Application
BRDT antibody detects BRDT protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with BRDT antibody at a dilution of 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
