Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using BRRN1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:500Application
BRRN1 antibody [N1N2], N-term detects BRRN1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BRRN1 protein stained by BRRN1 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | BRRN1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BRRN1 (non-SMC condensin I complex, subunit H)This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human BRRN1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC-P,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (85%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BRRN1 antibody, CAP-H antibody, HCAP-H antibody, NCAPH antibody, barren homolog 1 antibody, condensin complex subunit 2 antibody, barren homolog protein 1 antibody, XCAP-H homolog antibody, chromosome-associated protein H antibody, "non-SMC condensin I com |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) (4) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using BRRN1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:500
Application
BRRN1 antibody [N1N2], N-term detects BRRN1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BRRN1 protein stained by BRRN1 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
