Application
Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 7.5% SDS PAGE diluted at 1:3000Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using BRUNOL4 antibody at 1:500 dilution.| Product Name | BRUNOL4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BRUNOL4 (CUGBP Elav-like family member 4)Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BRUNOL4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (99%), Rat (99%), Zebrafish (86%), Xenopus laevis (97%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BRUNOL-4 antibody, BRUNOL4 antibody, CELF4 antibody, CUG-BP- and ETR-3-like factor 4 antibody, RNA-binding protein BRUNOL-4 antibody, "Bruno -like 4, RNA binding protein antibody", RNA-binding protein BRUNOL4 antibody, CELF-4 antibody, bruno-like protein 4 |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (2) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 7.5% SDS PAGE diluted at 1:3000
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using BRUNOL4 antibody at 1:500 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
