Application
Sample (30 ug of whole cell lysate) A: HeLa nucleus 10% SDS PAGE diluted at 1:1000Application
C10orf4 antibody detects C10orf4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C10orf4 protein stained by C10orf4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | C10orf4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C10orf4 (fragile site, folic acid type, rare, fra(10)(q23.3) or fra(10)(q24.2) candidate 1)The protein encoded by this gene is a nuclear phosphoprotein of unknown function. The 5' UTR of this gene is part of a CpG island and contains a tandem CGG repeat region that normally consists of 8-14 repeats but can expand to over 200 repeats. The expanded allele becomes hypermethylated and is not transcribed; however, an expanded repeat region has not been associated with any disease phenotype. This gene is found within the rare FRA10A folate-sensitive fragile site. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human C10orf4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, BovineMouse (90%), Rat (91%), Pig (93%), Rhesus Monkey (98%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C10orf4 antibody F26C11.1-like antibody FRA10A antibody FRA10AC1 antibody "rare folic acid-type fragile site FRA(10)(q23.3) candidate gene 1 antibody" fragile site 10q23.3 antibody protein FRA10AC1 antibody "fragile site folic acid type rare fr |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HeLa nucleus 10% SDS PAGE diluted at 1:1000
Application
C10orf4 antibody detects C10orf4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C10orf4 protein stained by C10orf4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HeLa , HeLa nucleus |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
