Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using C20orf7 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:2000Application
C20orf7 antibody [N3C3] detects C20orf7 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: C20orf7 protein stained by C20orf7 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | C20orf7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C20orf7 (chromosome 20 open reading frame 7)The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human C20orf7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, BovineMouse (92%), Rat (90%), Chicken (82%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ22324 antibody MGC90272 antibody bA526K24.2 antibody dJ842G6.1 antibody C20orf7 antibody "probable methyltransferase C20orf7 mitochondrial antibody" chromosome 20 open reading frame 7 antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (3) |
| Cellular Localization | Mitochondrion inner membrane |
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using C20orf7 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:2000
Application
C20orf7 antibody [N3C3] detects C20orf7 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: C20orf7 protein stained by C20orf7 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | NT2D1 , IMR32 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
