Application
ICC/IF analysis of HeLa cells using Carbonic Anhydrase III antibody (green) and DAPI (blue).Application
IHC-P analysis of rat testis using Carbonic Anhydrase III antibody at a dilution of 1:200.Application
WB analysis of extracts from various cell lines using Carbonic Anhydrase III antibody.| Product Name | CA3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CA3Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008] |
| Immunogen | Recombinant protein of human CA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAIII CA3 Car3 carbonic anhydrase III muscle specific |
|---|---|
| Target | CA3 |
| Molecular Weight(MW) | 30 kDa (note) |
Application
ICC/IF analysis of HeLa cells using Carbonic Anhydrase III antibody (green) and DAPI (blue).
Application
IHC-P analysis of rat testis using Carbonic Anhydrase III antibody at a dilution of 1:200.
Application
WB analysis of extracts from various cell lines using Carbonic Anhydrase III antibody.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
