Application
Sample (30 µg of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse heart 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CABC1 antibody at 1:500 dilution.Application
CABC1 antibody [N3C2], Internal detects CABC1 protein at mitochondria by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: CABC1 protein stained by CABC1 antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | CABC1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CABC1 (chaperone, ABC1 activity of bc1 complex homolog (S. pombe))This gene encodes a mitochondrial protein similar to yeast ABC1, which functions in an electron-transferring membrane protein complex in the respiratory chain. It is not related to the family of ABC transporter proteins. Expression of this gene is induced by the tumor suppressor p53 and in response to DNA damage, and inhibiting its expression partially suppresses p53-induced apoptosis. Alternatively spliced transcript variants have been found; however, their full-length nature has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CABC1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (93%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARCA2 antibody CABC1 antibody COQ8 antibody MGC4849 antibody SCAR9 antibody ADCK3 antibody "chaperone ABC1 activity of bc1 complex homolog antibody" coenzyme Q8 homolog antibody "chaperone activity of bc1 complex-like mitochondrial antibody" aar |
|---|---|
| Molecular Weight(MW) | 72 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 µg of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse heart 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CABC1 antibody at 1:500 dilution.
Application
CABC1 antibody [N3C2], Internal detects CABC1 protein at mitochondria by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: CABC1 protein stained by CABC1 antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | NT2D1 , IMR32 , mouse heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
