Application
Western blot analysis of Abl phosphorylation expression in Adriamycin treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Product Name | c-Abl (phospho Tyr412) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to c-Abl (phospho Tyr412)The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide derived from human Abl around the phosphorylation site of Tyr412 |
| Modification | p-Tyr412 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB 1:500-1:2000, IHC 1:50-1:200: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | phospho Abl (phospho Tyr412) (pTyr412) Phospho Y412 Abl (phospho Tyr412) JTK7 phospho Abl (phospho Tyr412) (Tyr412) Abl (phospho Tyr412) Phospho Y412 ABL1 Abl (phospho Tyr412) Tyr412 Phospho Anti |
|---|---|
| Target | Abl (phospho Tyr412) |
| Tissue Specificity | ABL1 |
Application
Western blot analysis of Abl phosphorylation expression in Adriamycin treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB 1:500-1:2000, IHC 1:50-1:200: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
