Application
ApplicationApplication
staining c-Abl in human placenta by Immunohistochemistry (FFPE-sections).| Product Name | c-Abl antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to c - AblThe c-Abl proto oncogene encodes a protein tyrosine kinase that is located in the cytoplasm and nucleus. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, the c-Abl proto oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) As the result of the fusion of c-Abl sequences from chromosome 9 to the Bcr gene on chromosome 22. The molecular consequence of this translocation is the generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase. |
| Immunogen | A synthetic peptide from the sequence adjacent to protein tyrosine kinase domain of human c-Abl. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PIPWB |
| IHC-P: Use at a dilution of 1/50 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 122.9 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | JTK7 ABL1 vabl P00519 ABL 25 cABL p150 v-abl 189980 c-ABL c Abl v abl |
|---|---|
| Target | c-Abl |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Application
Application
staining c-Abl in human placenta by Immunohistochemistry (FFPE-sections).| Positive Control | Placenta |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/50 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 122.9 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
