Application
Western blot analysis of Cadherin 19 expression in COLO205 (A); NIH3T3 (B) whole cell lysates.Application
Immunohistochemical analysis of Cadherin 19 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. w| Product Name | Cadherin 19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cadherin 19This gene is one of three related type II cadherin genes situated in a cluster on chromosome 18. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein containing five extracellular cadherin repeats. Loss of cadherins may be associated with cancer formation. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Aug 2012] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cadherin 19. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDH7L2 CDH7 CDH19 cadherin 19 type 2 |
|---|---|
| Target | Cadherin 19 |
| Tissue Specificity | Recognizes endogenous levels of Cadherin 19 protein. |
Application
Western blot analysis of Cadherin 19 expression in COLO205 (A); NIH3T3 (B) whole cell lysates.
Application
Immunohistochemical analysis of Cadherin 19 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. w| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
