Application
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody diluted at 1:1000.Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Calcineurin B antibody at 1:250 dilution.Application
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody diluted at 1:50000.| Product Name | Calcineurin B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calcineurin B (protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform)Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calcineurin B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, BovineXenopus laevis (97%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CALNB1 antibody CNB antibody CNB1 antibody PPP3R1 antibody protein phosphatase 2B regulatory subunit B alpha antibody "protein phosphatase 3 (formerly 2B) regulatory subunit B 19kDa alpha isoform (calcineurin B type I) antibody" "protein phosphat |
|---|---|
| Molecular Weight(MW) | 19 kDa (note) (2) |
Application
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody diluted at 1:1000.
Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Calcineurin B antibody at 1:250 dilution.
Application
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody diluted at 1:50000.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 , mouse fetal brain , rat brain , *MEF |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
