Application
Western blot analysis of CALM1 Antibody (C-term) in Hela cell line lysates (35ug/lane). CALM1 (arrow) was detected using the purified Pab.(2ug/ml)Application
CALM1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of CALM1 Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
CALM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | CALM1 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CALM1 (C-term)Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP11 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. |
| Immunogen | KLH conjugated synthetic peptide between 120~149 amino acids from the C-terminal region of human CALM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Calmodulin CALM CaM CALM1 CALM2 CALM3 CALM1 CAM1 |
|---|---|
| Target | CALM1 |
| Molecular Weight(MW) | 16.838 kDa (note) |
| Cellular Localization | Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. |
Application
Western blot analysis of CALM1 Antibody (C-term) in Hela cell line lysates (35ug/lane). CALM1 (arrow) was detected using the purified Pab.(2ug/ml)
Application
CALM1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of CALM1 Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
CALM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
