Application
Western blot analysis: Lane 1: MWM Lane 2: Vero Lane 3: 3T3 Lane 4: PC-12 Lane 5: HeLa.Application
Immunohistochemistry analysis of human placenta tissue stained with Calnexin, pAb at 5µg/ml.| Product Name | Calnexin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Calnexin - ER MarkerCalnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). |
| Immunogen | Synthetic peptide: TAPPSSPKVTYKAPVPTGE conjugated to KLH, corresponding to amino acids 50-68 of Human Calnexin |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHCIPWB |
| ICC: Use at a dilution of 1/200. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/5000. (This dilution was sufficient for detection of Calnexin in 20μg of HeLa heat shocked cell lysate). Detects a band of approximately 90 kDa. (predicted molecular weight: 71 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseMonkeyPigRatGuinea PigHamsterQuailRabbitSheepXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P90 IP90 821 CANX 114217 CNX P27824 Calnexin |
|---|---|
| Target | Calnexin |
| Cellular Localization | Endoplasmic reticulum |
Application
Western blot analysis: Lane 1: MWM Lane 2: Vero Lane 3: 3T3 Lane 4: PC-12 Lane 5: HeLa.
Application
Immunohistochemistry analysis of human placenta tissue stained with Calnexin, pAb at 5µg/ml.| Positive Control | Heat Shocked HeLa Cell Lysate |
|---|---|
| Application Notes | ICC: Use at a dilution of 1/200. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/5000. (This dilution was sufficient for detection of Calnexin in 20μg of HeLa heat shocked cell lysate). Detects a band of approximately 90 kDa. (predicted molecular weight: 71 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: Whole Serum |
