Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Calpain 2 antibody [N2C1-2], Internal detects Calpain 2 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Calpain 2 antibody [N2C1-2], Internal diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Calpain 2 antibody at 1:500 dilution.| Product Name | Calpain 2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calpain 2 (calpain 2, (m/II) large subunit)The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calpain 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, BovineRat (91%), Pig (94%), Rabbit (93%), Chicken (86%), Sheep (94%), Rhesus Monkey (98%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CANP2 antibody CANPL2 antibody CANPml antibody FLJ39928 antibody mCANP antibody CAPN2 antibody "calpain large polypeptide L2 antibody" "calpain 2 large [catalytic] subunit antibody" calpain-2 large subunit antibody "calpain 2 large subunit anti |
|---|---|
| Molecular Weight(MW) | 80 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Cell membrane |
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Calpain 2 antibody [N2C1-2], Internal detects Calpain 2 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Calpain 2 antibody [N2C1-2], Internal diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Calpain 2 antibody at 1:500 dilution.| Positive Control | 293T , A431 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
