Application
Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using Calpain 3 antibody at 1:200 dilution.| Product Name | Calpain 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Calpain 3 (calpain 3, (p94))Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calpain 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Sheep, BovineMouse (86%), Rat (89%), Pig (88%), Sheep (87%), Bovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CANP3 antibody CANPL3 antibody LGMD2 antibody LGMD2A antibody MGC10767 antibody MGC11121 antibody MGC14344 antibody MGC4403 antibody nCL-1 antibody p94 antibody CAPN3 antibody new calpain 1 antibody calpain-3 antibody "calpain p94 large [cata |
|---|---|
| Target | Calpain 3 |
| Molecular Weight(MW) | 94 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Calpain 3 antibody at 1:200 dilution.| Positive Control | H1299 , HeLa , HepG2 , Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
