Application
Western blot analysis of Calponin-1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). CNN1 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of NCI-H292 cells using Calponin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human lung carcinoma with Calponin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Calponin-1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Calponin-1 (N-term)Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. |
| Immunogen | KLH conjugated synthetic peptide between 17~47 amino acids from the N-terminal region of human Calponin-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Calponin1 Basic calponin CNN1 Calponin H1 smooth muscle Calponin-1 Calponin 1 |
|---|---|
| Target | Calponin-1 |
| Molecular Weight(MW) | 33.17 kDa (note) |
Application
Western blot analysis of Calponin-1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). CNN1 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of NCI-H292 cells using Calponin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with Calponin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
