Application
IHC image using - Detection of Calretinin by IHC in human mesothelioma| Product Name | Calretinin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CalretininThe antibody is specific against a calcium binding protein called calretinin. This antibody reacts specifically with calretinin in tissue originating from human, rat and mouse. Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas. |
| Immunogen | Recombinant full length mouse Calretinin protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature or overnight at 4 degrees Celsius. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Calretinin 114051 CR 794 CAL2 12308 CALB2 P22676 |
|---|---|
| Target | Calretinin |
| Cellular Localization | Cytoplasmic |
Application
IHC image using - Detection of Calretinin by IHC in human mesothelioma| Positive Control | Mesothelioma |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature or overnight at 4 degrees Celsius. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
