Application
Calretinin antibody detects Calretinin protein in the amacrine cells by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Calretinin protein stained by Calretinin antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calretinin antibody at 1:200 dilution.Application
Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Calretinin antibody dilution: 1:500.Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin antibody at 1:250 dilution.Application
Sample (30 µg of whole cell lysate) A: A431 12% SDS PAGE Calretinin antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Calretinin antibody detects CALB2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human CALB2-transfected 293T cells 12% SDS-PAGE Calretinin antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Calretinin antibody detects Calretinin protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calretinin protein stained by Calretinin antibody diluted at 1:200. Blue: Hoechst 33342 staining.Application
Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Calretinin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calretinin (calbindin 2)This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Three alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Chicken, BovinePig (96%), Chicken (89%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAB29 antibody CAL2 antibody CR antibody CALB2 antibody calbindin 2 (29kD calretinin) antibody 29 kDa calbindin antibody calbindin D29K antibody calretinin antibody calbindin 2 antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa (note) |
Application
Calretinin antibody detects Calretinin protein in the amacrine cells by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Calretinin protein stained by Calretinin antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calretinin antibody at 1:200 dilution.
Application
Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Calretinin antibody dilution: 1:500.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin antibody at 1:250 dilution.
Application
Sample (30 µg of whole cell lysate) A: A431 12% SDS PAGE Calretinin antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Calretinin antibody detects CALB2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human CALB2-transfected 293T cells 12% SDS-PAGE Calretinin antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Calretinin antibody detects Calretinin protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calretinin protein stained by Calretinin antibody diluted at 1:200. Blue: Hoechst 33342 staining.
Application
Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , human CALB2-transfected 293T cells , mouse brain, rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
