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IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. We suggest an incubation period of 30 minutes at room temperature or overnight at 4 degrees Celsius. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.:
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