Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calsequestrin 1 antibody diluted at 1:1000.| Product Name | Calsequestrin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calsequestrin 1 (calsequestrin 1 (fast-twitch, skeletal muscle))The protein encoded by this gene is a mitochondrial calcium-binding protein located in the luminal space of the terminal cisternae of the sarcoplasmic reticulum. The protein binds and putatively stores calcium ions. The protein is absent in patients with Duchenne and Becker types of muscular dystrophy. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calsequestrin 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rabbit, BovinePig (96%), Rabbit (96%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CASQ antibody PDIB1 antibody CASQ1 antibody calmitine antibody "calsequestrin skeletal muscle isoform antibody" calmitin antibody calsequestrin-1 antibody "calsequestrin 1 (fast-twitch skeletal muscle) antibody" |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (2) |
| Cellular Localization | Sarcoplasmic reticulum lumen , Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calsequestrin 1 antibody diluted at 1:1000.| Positive Control | HepG2 , mouse muscle , rat muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
