Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CAPG antibody at 1:500 dilution.Application
CAPG antibody [N3C3] detects CAPG protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CAPG protein stained by CAPG antibody [N3C3] diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:2000Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE CAPG antibody diluted at 1:1000| Product Name | CAPG antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CAPG (capping protein (actin filament), gelsolin-like)This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CAPG. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (93%), Rhesus Monkey (99%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AFCP antibody MCP antibody CAPG antibody macrophage capping protein antibody actin-regulatory protein CAP-G antibody macrophage-capping protein antibody gelsolin-like capping protein antibody actin regulatory protein CAP-G antibody "capping protein |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) (5) |
| Cellular Localization | Cytoplasm , Nucleus , Melanosome |
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CAPG antibody at 1:500 dilution.
Application
CAPG antibody [N3C3] detects CAPG protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CAPG protein stained by CAPG antibody [N3C3] diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:2000
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE CAPG antibody diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
