Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase I antibody diluted at 1:1000.| Product Name | Carbonic Anhydrase I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Carbonic Anhydrase I (carbonic anhydrase I)Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Carbonic Anhydrase I. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Dog, ChimpanzeeHuman (100%), Dog (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | carbonic anhydrase I HEL-S-11 CA1 CA-I Car1 CAB |
|---|---|
| Target | Carbonic Anhydrase I |
| Molecular Weight(MW) | 29 kDa (note) (5) |
| Cellular Localization | Cytoplasm |
Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase I antibody diluted at 1:1000.| Positive Control | mouse spleen |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
