Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:5000Application
CARD6 antibody [N1N2], N-term detects CARD6 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CARD6 protein stained by CARD6 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | CARD6 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CARD6 (caspase recruitment domain family, member 6)This gene encodes a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CARD6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CINCIN1 antibody CARD6 antibody caspase recruitment domain-containing protein 6 antibody caspase recruitment domain protein 6 antibody CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation antibody "caspase recruitment domain family |
|---|---|
| Molecular Weight(MW) | 116 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:5000
Application
CARD6 antibody [N1N2], N-term detects CARD6 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CARD6 protein stained by CARD6 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
