Application
ICC/IF staining of methanol- fixed HeLa cells using CARM1 (phospho Ser228) antibody.Application
WB analysis of extracts from untreated and EGF-treated A431 cells using CARM1 (phospho Ser228) antibody.| Product Name | CARM1 (phospho Ser228) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CARM1 (phospho Ser228)Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM, Mar 2008] |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S228 of human CARM1 |
| Modification | p-Ser228 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: 1:500 - 1:2000. IF: 1:100 - 1:200.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CARM1 PRMT4 coactivator-associated arginine methyltransferase 1 |
|---|---|
| Target | CARM1 (phospho Ser228) |
| Molecular Weight(MW) | 63 kDa (note) |
Application
ICC/IF staining of methanol- fixed HeLa cells using CARM1 (phospho Ser228) antibody.
Application
WB analysis of extracts from untreated and EGF-treated A431 cells using CARM1 (phospho Ser228) antibody.| Application Notes | WB: 1:500 - 1:2000. IF: 1:100 - 1:200.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
