Application
Immunofluorescence staining of methanol-fixed HeLa cells using CARM1 antibody (, Red).Application
Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using CARM1 antibody (, Line 1 and 2) and CARM1 (Phospho-Ser228) antibody (11331, Line 3 and 4).| Product Name | CARM1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CARM1Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. |
| Immunogen | Peptide sequence around aa.226~230 (V-K-S-N-N) derived from CARM1 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PRMT4 CARM1 protein arginine N-methyltransferase 4 histone-arginine methyltransferase CARM1 coactivator-associated arginine methyltransferase 1 |
|---|---|
| Target | CARM1 |
| Molecular Weight(MW) | 63 (note) |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using CARM1 antibody (, Red).
Application
Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using CARM1 antibody (, Line 1 and 2) and CARM1 (Phospho-Ser228) antibody (11331, Line 3 and 4).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
