Application
Sample (30 µg of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using Casein Kinase 2 beta antibody at 1:100 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Casein Kinase 2 beta antibody at 1:500 dilution.Application
Casein Kinase 2 beta antibody detects Casein Kinase 2 beta protein at nucleus and cytosol on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. Casein Kinase 2 beta antibody dilution: 1:500.| Product Name | Casein Kinase 2 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Casein Kinase 2 beta (casein kinase 2, beta polypeptide)This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Casein Kinase 2 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, BovineRat (100%), Zebrafish (100%), Xenopus laevis (100%), Pig (100%), Rabbit (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CK2B antibody CK2N antibody CSK2B antibody G5A antibody MGC138222 antibody MGC138224 antibody CSNK2B antibody CK II beta antibody Casein kinase II beta subunit antibody "alternative name: G5a phosvitin antibody" phosvitin antibody protein G5a a |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) (2) |
Application
Sample (30 µg of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using Casein Kinase 2 beta antibody at 1:100 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Casein Kinase 2 beta antibody at 1:500 dilution.
Application
Casein Kinase 2 beta antibody detects Casein Kinase 2 beta protein at nucleus and cytosol on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. Casein Kinase 2 beta antibody dilution: 1:500.| Positive Control | MCF-7 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
