Application
Immunohistochemical staining of human heart tissue using Caspase-1 antibody at 2 ug/mL.Application
Western blot analysis of Caspase-1 in human heart cell lysate with Caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.| Product Name | Caspase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 1This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012] |
| Immunogen | Caspase-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 0.5 - 2ug/ml. IHC-P: 2 ug/ml. IF: 10 ug/ml: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | caspase 1 apoptosis-related cysteine peptidase CASP1 ICE P45 IL1BC |
|---|---|
| Target | Caspase 1 |
| Tissue Specificity | Depending on cell lines or tissues used, other cleavage products may be observed. |
Application
Immunohistochemical staining of human heart tissue using Caspase-1 antibody at 2 ug/mL.
Application
Western blot analysis of Caspase-1 in human heart cell lysate with Caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.| Application Notes | WB: 0.5 - 2ug/ml. IHC-P: 2 ug/ml. IF: 10 ug/ml: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
