Application
Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 1 alpha antibody at 1:500 dilution.Application
Treated (+) THP-1 conditioned medium (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 1 antibody [N1N3] diluted at 1:1000.Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:5000Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Caspase 1 alpha antibody at 1:100 dilution.| Product Name | Caspase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 1 alpha (caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase))This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Caspase 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ICE antibody IL1BC antibody P45 antibody CASP1 antibody CASP1 nirs variant 1 antibody IL-1BC antibody caspase-1 antibody interleukin 1-B converting enzyme antibody IL1B-convertase antibody IL-1 beta-converting enzyme antibody "caspase 1 apoptosi |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 1 alpha antibody at 1:500 dilution.
Application
Treated (+) THP-1 conditioned medium (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 1 antibody [N1N3] diluted at 1:1000.
Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:5000
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Caspase 1 alpha antibody at 1:100 dilution.| Positive Control | A549 , HeLa , HepG2 , HCT116 , THP-1 conditioned medium (500 ng/ml LPS treatment for 4 hr and 1 mM ATP treatment for 1.5hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
