Application
Western blot analysis of Caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody at 1μg/ml.| Product Name | Caspase 10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Caspase 10Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3protease family. A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch41,2 and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is therefore involved in the CD95 and TNFR1 inducedapoptosis1. Caspase-10 °Cleaves and activates caspase-3,-4, -6, -7, -8 and -9, which causes the proteolytic cleavage of many key proteins such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death (ref 3). Caspase-10 is expressed in many tissues and cell lines (ref 1,2). |
| Immunogen | Synthetic peptide, corresponding to amino acids 505-521 of Human Caspase 10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| This polyclonal antibody can be used for detection ofcaspase-10 by Western blot at 1:500 to 1:1000 dilution anda 59 kDa band can be detected.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Gene Synonyms | Caspase 10 ALPS2 Caspase10 137215 MCH4 CASP10 FLICE2 Q92851 843 |
|---|---|
| Target | Caspase 10 |
| Tissue Specificity | This antibody only recognizes FLICE2 form of caspase-10. |
Application
Western blot analysis of Caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody at 1μg/ml.| Positive Control | HeLa whole cell lysate. |
|---|---|
| Application Notes | This polyclonal antibody can be used for detection ofcaspase-10 by Western blot at 1:500 to 1:1000 dilution anda 59 kDa band can be detected.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
