Application
Western blot analysis of caspase-10 expression in Jurkat cells treated with camptothecin.| Product Name | Caspase 10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 10Caspase family of cysteine proteases play a key role in apoptosis. Caspase-10 is expressed in many tissues and cell lines, and has been identified as caspase-10/a (short form) and caspase-10/b (long form), respectively. Similar as other caspases, caspase-10 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Activated caspase-10 activates caspase-3,-4,-6,-7,-8, and –9, which are then involved in proteolytic cleavage of many cellular proteins. Caspase-10 has been shown involving in the Fas and TNFR1 induced apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the proform (~55 kDa), intermediates (30 kDa and 34 kDa), and cleaved form (~20 kDa) of caspase-10 on SDS-PAGE immunoblots.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ALPS2 ICElike apoptotic protease4 MCH4 ICElike apoptotic protease 4 CASP10 FLICE2 CASP-10 ICE-like apoptotic protease 4 Caspase10 CASP 10 ICE like apoptotic protease 4 |
|---|---|
| Target | Caspase 10 |
| Tissue Specificity | This antibody is specific for Caspase-10. |
Application
Western blot analysis of caspase-10 expression in Jurkat cells treated with camptothecin.| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the proform (~55 kDa), intermediates (30 kDa and 34 kDa), and cleaved form (~20 kDa) of caspase-10 on SDS-PAGE immunoblots.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
