Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000Application
Caspase 14 antibody detects Caspase 14 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 14 antibody at a dilution of 1:1000.| Product Name | Caspase 14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 14 (caspase 14, apoptosis-related cysteine peptidase)This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Caspase 14. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC119078 antibody MGC119079 antibody CASP14 antibody "caspase 14 apoptosis-related cysteine protease antibody" caspase-14 antibody apoptosis-related cysteine protease antibody CASP-14 antibody "caspase 14 apoptosis-related cysteine peptidase anti |
|---|---|
| Target | Caspase 14 |
| Molecular Weight(MW) | 28 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000
Application
Caspase 14 antibody detects Caspase 14 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 14 antibody at a dilution of 1:1000.| Positive Control | A431 , PC-3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
