Application
Western blot analysis of active Caspase 3 in camptothecin (2 μM) treated Jurkat cells.WB
A–E, Western blotting evaluations of Hsp70 (A), cleaved caspase-3 (B), p-Akt and Akt (C), p-JNK and JNK (D), and p-ERK and ERK (E) levels in the myocardium.| Product Name | Caspase 3 (active) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 3 (active)Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity-purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. |
| Immunogen | Available on request |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-FIHC-PWB |
| For IHC-P: Use at a concentration of 10 - 20 μg/ml. : |
|
| Species Reactivity | FerretHumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 836 P42574 Caspase 3 active CASP3 SCA1 Caspase3 active CPP32B CPP32 SCA-1 600636 SCA 1 Caspase 3 (Active) Active Caspase 3 |
|---|---|
| Target | Caspase 3 (active) |
| Tissue Specificity | The antibody preferentially recognizes the p17 fragment of the active Caspase 3 . |
Application
Western blot analysis of active Caspase 3 in camptothecin (2 μM) treated Jurkat cells.
WB
A–E, Western blotting evaluations of Hsp70 (A), cleaved caspase-3 (B), p-Akt and Akt (C), p-JNK and JNK (D), and p-ERK and ERK (E) levels in the myocardium.| Positive Control | Rat brain,rat foetus, mouse foetus (day 9)(head, heart) (see reference Mirkes et al) |
|---|---|
| Application Notes | For IHC-P: Use at a concentration of 10 - 20 μg/ml. : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.4 containing 50% glycerol, 1 mg/ml BSA and 0.02% thimerosal |
