Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Caspase 3 (Cleaved-Asp175) Antibody. The picture on the right is treated with the synthesized peptide.Application
Western blot analysis of extracts from HeLa cells, treated with Etoposide 25uM 60', using Caspase 3 (Cleaved-Asp175) antibody . The lane on the right is treated with the synthesized peptide.| Product Name | Caspase 3 (cleaved Asp175) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 3 (cleaved Asp175)Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. |
| Immunogen | The immunogen corresponding to a region within amino acids 157 and 206 of human Caspase 3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| Recommended Starting Dilutions: For WB: Use at a dilution of 1:500-1:1000. For IHC-P: Use at a dilution of 1:50-1:100. For ELISA: Use at a dilution of 1:20000. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ICE3 CPP-32 Apopain precursor SCA1 Yama protein Caspase-3 SREBP cleavage activity1 CASP3 Cysteine protease CPP32 Caspase3 SREBP cleavage activity 1 Caspase 3 CASP-3 SCA-1 CPP32 SCA 1 CPP 32 CASP 3 Caspase 3 (cleaved D175) Caspase 3 (clea |
|---|---|
| Target | Caspase 3 |
Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Caspase 3 (Cleaved-Asp175) Antibody. The picture on the right is treated with the synthesized peptide.
Application
Western blot analysis of extracts from HeLa cells, treated with Etoposide 25uM 60', using Caspase 3 (Cleaved-Asp175) antibody . The lane on the right is treated with the synthesized peptide.| Application Notes | Recommended Starting Dilutions: For WB: Use at a dilution of 1:500-1:1000. For IHC-P: Use at a dilution of 1:50-1:100. For ELISA: Use at a dilution of 1:20000. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C |
| Storage Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
