Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Caspase 3 (Cleaved-Asp175) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | Caspase 3 (cleaved Asp175) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 3 (cleaved Asp175)Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-P |
| IHC: 1:50~1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ICE3 CPP-32 Apopain precursor SCA1 Yama protein Caspase-3 SREBP cleavage activity1 CASP3 Cysteine protease CPP32 Caspase3 SREBP cleavage activity 1 Caspase 3 CASP-3 SCA-1 CPP32 SCA 1 CPP 32 CASP 3 Caspase 3 (cleaved D175) Caspase 3 (clea |
|---|---|
| Target | Caspase 3 |
| Molecular Weight(MW) | 20 and 35 kDa (note) |
| Tissue Specificity | Caspase 3 (Cleaved-Asp175) Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175. |
Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Caspase 3 (Cleaved-Asp175) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IHC: 1:50~1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
