Application
Western blot analysis of extracts from 293 cells, treated with Etoposide 25uM 60', using Caspase 3 (Cleaved-Asp175) Antibody. The lane on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 3 (Cleaved-Asp175) antibody . The picture on the right is treated with the synthesized peptide.| Product Name | Caspase 3 (cleaved Asp175) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 3 (cleaved Asp175)Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. |
| Immunogen | The immunogen was produced against synthesized peptide(126-175a.a) derived from human Caspase 3 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: ELISA:1:5000~20000 IHC(P):1:50~1:100 WB:1:500~1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ICE3 CPP-32 Apopain precursor SCA1 Yama protein Caspase-3 SREBP cleavage activity1 CASP3 Cysteine protease CPP32 Caspase3 SREBP cleavage activity 1 Caspase 3 CASP-3 SCA-1 CPP32 SCA 1 CPP 32 CASP 3 Caspase 3 (cleaved D175) |
|---|---|
| Target | Caspase 3 |
Application
Western blot analysis of extracts from 293 cells, treated with Etoposide 25uM 60', using Caspase 3 (Cleaved-Asp175) Antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 3 (Cleaved-Asp175) antibody . The picture on the right is treated with the synthesized peptide.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: ELISA:1:5000~20000 IHC(P):1:50~1:100 WB:1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable at -20°C |
| Storage Buffer | PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol |
