Application
IHC-P analysis of human tonsil stained using Caspase 3 antibody.Application
Formalin fixed paraffin embedded human tonsil stained with Caspase 3 antibody (cat. # ).| Product Name | Caspase 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Caspase 3The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. caspases 8, 9 and 10). The processed form of caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active caspase 3 proteolytically cleaves and activates other caspases (e.g. caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-P |
| Immunohistochemistry (frozen sections): Use at a dilution of 1:50 - 1:100 for 30mins at RT (ABC method). Immunohistochemistry (formalin-fixed paraffin-embedded sections): Use at a dilution of 1:50 - 1:100 for 30mins at RT (ABC method). Perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogMonkeyPigRatHamsterRabbitSheepBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | 836 P42574 CASP3 Caspase3 SCA1 Caspase 3 CPP32B CPP32 SCA-1 600636 SCA 1 |
|---|---|
| Target | Caspase 3 |
| Tissue Specificity | This antibody reacts with a 32 kDa protein |
| Cellular Localization | Cytoplasmic |
Application
IHC-P analysis of human tonsil stained using Caspase 3 antibody.
Application
Formalin fixed paraffin embedded human tonsil stained with Caspase 3 antibody (cat. # ).| Positive Control | Tonsil |
|---|---|
| Application Notes | Immunohistochemistry (frozen sections): Use at a dilution of 1:50 - 1:100 for 30mins at RT (ABC method). Immunohistochemistry (formalin-fixed paraffin-embedded sections): Use at a dilution of 1:50 - 1:100 for 30mins at RT (ABC method). Perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH 7.4 containing 0.2% BSA and 0.09% sodium azide |
