Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 6 (cleaved-Asp179) antibody.Application
Western blot analysis of extracts from 293 cells, treated with Etoposide (25uM, 60mins), using Caspase 6 (cleaved-Asp179) antibody.| Product Name | Caspase 6 (Cleaved Asp179) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 6 (Cleaved Asp179)This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| WB: 1:500 - 1:1000. IHC: 1:50 - 1:100. ELISA: 1:40000: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | caspase 6 apoptosis-related cysteine peptidase CASP6 MCH2 |
|---|---|
| Target | Caspase 6 (Cleaved Asp179) |
| Tissue Specificity | Caspase 6 (Cleaved-Asp179) Antibody detects endogenous levels of fragment of activated Caspase 6 resulting from cleavage adjacent to Asp179. |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 6 (cleaved-Asp179) antibody.
Application
Western blot analysis of extracts from 293 cells, treated with Etoposide (25uM, 60mins), using Caspase 6 (cleaved-Asp179) antibody.| Application Notes | WB: 1:500 - 1:1000. IHC: 1:50 - 1:100. ELISA: 1:40000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable at -20°C for at least 1 year. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
