Rabbit Polyclonal antibody to Caspase 6This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. [provided by RefSeq, Jul 2008]
Immunogen
Caspase-6 antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human Caspase-6.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAICC/IFWB
WB: 0.5 - 2 ug/ml. ICC: 2 ug/ml. IF: 20 ug/ml:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.
Application
Application
Immunocytochemistry of caspase-6 in Jurkat cells with caspase-6 antibody at 2 ug/mL.
Application
Western blot analysis of caspase-6 in Jurkat cell lysate with caspase-6 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Application Notes
WB: 0.5 - 2 ug/ml. ICC: 2 ug/ml. IF: 20 ug/ml:
Additional Information
Form
Liquid
Storage Instructions
Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.