Application
Western blot analysis of Jurkat cells induced with 2mM of Camptothecin for 6 hours.| Product Name | Caspase 7 (active) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 7 (active)Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 7 has been identified as a major contributor to the execution of apoptosis. Caspase 7 is cleaved by many enzymes, including caspase 3, 6, 8, 9 and granzyme B. Active caspase 7 consists of a heterotetramer of two large and two small subunits. As an effector caspase, caspase 7 cleaves many of the same substrates as caspase 3, including poly (ADP-ribose) polymerase (PARP). The active caspase 7 antibody provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. |
| Immunogen | Available on request. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| FACS: Use at an assay dependent dilution. IHC-Fr: Use at a concentration of 10 - 20 μg/ml. IP: Use at a concentration of 10 - 20 μg/ml. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 20 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ICELAP3 Caspase7 active ICE-LAP3 CMH-1 CASP7 CMH1 601761 840 Caspase 7 active P55210 MCH3 CMH 1 ICE LAP3 Caspase 7 (Active) Active Caspase 7 |
|---|---|
| Target | Caspase 7 (active) |
| Tissue Specificity | This antibody recognizes only the large subunit of cleaved caspase 7. The antibody does not recognize full length caspase 7 or any other caspase. |
Application
Western blot analysis of Jurkat cells induced with 2mM of Camptothecin for 6 hours.| Application Notes | FACS: Use at an assay dependent dilution. IHC-Fr: Use at a concentration of 10 - 20 μg/ml. IP: Use at a concentration of 10 - 20 μg/ml. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 20 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 1 mg/ml BSA, 1.5 mM sodium azide and 50% glycerol |
