Application
Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 24h, using Caspase 7 (p20,Cleaved-Asp198) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | Caspase 7 (cleaved Asp198) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 7 (cleaved Asp198)Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 7. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MCH3 ICELAP3 CASP7 CMH1 CASP7 (cleaved D198) CASP7 (cleaved Asp198) CASP7 cleaved D198 CASP7 cleaved Asp198 Cleaved CASP7 (D198) Cleaved CASP7 (Asp198) |
|---|---|
| Target | CASP7 |
| Molecular Weight(MW) | 20 kDa (note) |
| Tissue Specificity | Caspase 7 (p20,Cleaved-Asp198) Antibody detects endogenous levels of fragment of activated Caspase 7 resulting from cleavage adjacent to Asp198. |
Application
Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 24h, using Caspase 7 (p20,Cleaved-Asp198) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
