Rabbit Polyclonal antibody to Caspase 7This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Immunogen
Caspase-7 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-7.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIHC-PWB
WB: 0.5 - 1 ug/ml. IHC-P: 2 ug/ml:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Depending on cell lines or tissues used, other cleavage products may be observed.
Application
Application
Immunohistochemical staining of human skeletal muscle using Caspase-7 antibody at 2 ug/mL.
Application
Western blot analysis of Caspase-7 in human skeletal muscle cell lysate with Caspase-7 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Application Notes
WB: 0.5 - 1 ug/ml. IHC-P: 2 ug/ml:
Additional Information
Form
Liquid
Storage Instructions
Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.