Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Caspase 7 p11 subunit antibody at 1:500 dilution.Application
Wild-type (WT) and Caspase 7 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 p11 subunit antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000| Product Name | Caspase 7 p11 subunit antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 7 p11 subunit (caspase 7, apoptosis-related cysteine peptidase)This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Caspase 7 p11 subunit. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevisMouse (91%), Rat (91%), Zebrafish (82%), Xenopus laevis (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CMH-1 antibody ICE-LAP3 antibody MCH3 antibody CASP7 antibody "caspase 7 apoptosis-related cysteine protease antibody" CASP-7 antibody Lice2 alpha/beta/gamma antibody caspase 7 isoform delta antibody apoptotic protease MCH-3 antibody caspase-7 an |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (8) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Caspase 7 p11 subunit antibody at 1:500 dilution.
Application
Wild-type (WT) and Caspase 7 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 p11 subunit antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
