Application
Western blot analysis of caspase-8 expression in mouse intestine and rat kidney tissue lysate.| Product Name | Caspase 8 (active) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 8 (active)Caspase-8, a member of the caspase-family of proteases, plays a key role in mediating Fas (CD95) and TNF induced apoptosis. Caspase-8 is synthesized as inactive pro-enzyme and activation of the enzyme involves proteolytic cleavage that leads to the release of the active p18 and p10 subunits. Activated caspase-8 is able to cleave and activate downstream caspases, such as caspase-3, -6, -7 and a death agonist member of the Bcl-2/Bcl-xL family, Bid, leading to apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml) in samples from mouse and rat origins. However, the optimal conditions should be determined individually. The antibody detects the 25 kDa large subunit of caspase-8. It does not detect the full-length of caspase-8.: |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EC 3.4.22.61 MGC78473 MACH procaspase8 CASP-8 procaspase-8 EC3.4.22.61 Caspase8 CASP8 MCH5 ALPS2B FLICE CAP4 CASP 8 procaspase 8 Caspase-8 (Active) Active Caspase-8 |
|---|---|
| Target | Caspase 8 (active) |
| Tissue Specificity | This antibody is specific for Caspase-8 (Active). |
Application
Western blot analysis of caspase-8 expression in mouse intestine and rat kidney tissue lysate.| Application Notes | Western blotting (0.5-4 µg/ml) in samples from mouse and rat origins. However, the optimal conditions should be determined individually. The antibody detects the 25 kDa large subunit of caspase-8. It does not detect the full-length of caspase-8.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at −20°C .The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 0.5% BSA, and 0.01% thimerosal |
