Application
Western blot analysis of Caspase 8 (pY380) expression in HEK293T PMA-treated (A); Jurkat etoposide-treated (B); HeLa PMA-treated (C); H9C2 PMA-treated (D) whole cell lysates.| Product Name | Caspase 8 (phospho Tyr380) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 8 (phospho Tyr380)This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 8. The exact sequence is proprietary. |
| Modification | p-Tyr380 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Casp-8 caspase 8 apoptosis-related cysteine peptidase MCH5 CAP4 CASP8 MACH FLICE ALPS2B |
|---|---|
| Target | Caspase 8 |
| Tissue Specificity | Recognizes endogenous levels of Caspase 8 (pY380) protein. |
Application
Western blot analysis of Caspase 8 (pY380) expression in HEK293T PMA-treated (A); Jurkat etoposide-treated (B); HeLa PMA-treated (C); H9C2 PMA-treated (D) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
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| Form | Liquid |
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| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
