Application
Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 ug/mL.Application
Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at 1ug/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 ug/mL.| Product Name | Caspase 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 8This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008] |
| Immunogen | Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| IF: 20 ug/mL: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MACH Casp-8 FLICE caspase 8 apoptosis-related cysteine peptidase ALPS2B CASP8 MCH5 CAP4 |
|---|---|
| Target | Caspase 8 |
| Tissue Specificity | Depending on cell lines or tissues used, either full-length or other cleavage products may be observed. |
Application
Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 ug/mL.
Application
Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at 1ug/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 ug/mL.| Application Notes | IF: 20 ug/mL: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
