Application
WB analysis on Jurkat cell lysate 1.) Untreated 2.) Etoposide treated| Product Name | Caspase 9 (active) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 9 (active)Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. |
| Immunogen | Available on request |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| ICC: Use at a concentration of 10 - 20 μg/ml. IP: Use at a concentration of 10 - 20 μg/ml. WB: Use at a concentration of 0.5 - 1 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 602234 P55211 ICE-LAP6 842 CASPASE9c Caspase9 active APAF3 Caspase 9 active MCH6 CASP9 CASPASE-9c ICELAP6 APAF-3 AI115399 AW493809 Caspase-9 APAF 3 ICE LAP6 Caspase 9 CASPASE 9c Caspase9 Caspase 9 (Active) Active Caspase 9 |
|---|---|
| Target | Caspase 9 (active) |
| Tissue Specificity | This antibody recognizes only the cleaved caspase 9 (37 kDa). It does not recognize full-length caspase 9 or any other caspase. |
Application
WB analysis on Jurkat cell lysate 1.) Untreated 2.) Etoposide treated| Application Notes | ICC: Use at a concentration of 10 - 20 μg/ml. IP: Use at a concentration of 10 - 20 μg/ml. WB: Use at a concentration of 0.5 - 1 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 1 mg/ml BSA, 1.5 mM sodium azide and 50% glycerol |
