Application
Western blot analysis of extracts from 293 cells, treated with Etoposide 25uM 60', using Caspase 9 (Cleaved-Asp315) Antibody. The lane on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 (Cleaved-Asp315) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | Caspase 9 (cleaved Asp315) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 9 (cleaved Asp315)Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
| Immunogen | The amino acid range of this immunogen is aa285-315 of human Caspase 9 with accession number P55211 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| IHC: 1:50~1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Caspase9 inhibitor of CAD H13 Caspase 9 DFF-45 DFF1 DNAation factor45 kDa subunit DNAation factor 45 kDa subunit DFF45 ICAD DFF 45 Caspase 9 (cleaved D315) Caspase 9 (cleaved Asp315) Caspase 9 cleaved D315 Caspase 9 cleaved Asp315 Cleaved Ca |
|---|---|
| Target | Caspase 9 |
| Molecular Weight(MW) | 37 kDa (note) |
| Tissue Specificity | Caspase 9 (Cleaved-Asp315) Antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp315. |
Application
Western blot analysis of extracts from 293 cells, treated with Etoposide 25uM 60', using Caspase 9 (Cleaved-Asp315) Antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 (Cleaved-Asp315) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IHC: 1:50~1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
